RNA-seq Variant Frame Analyzer
Input reference and variant sequences to assess reading frame disruption and protein impact
Input
Sequence / Variant Read
DNA (T) or RNA (U) accepted — paste directly from IGV
Reference Sequence
optional — enables side-by-side comparison
Direction:
Forward (5'→3')
Reverse complement (3'→5')
Both directions
Analyze
Clear
Load example:
— select —
Frameshift insertion (1 bp)
In-frame deletion (3 bp)
Missense SNV
Nonsense SNV (premature stop)
Synonymous SNV (silent)
Normal codon
Changed AA
Stop codon
New stop (variant only)
Frameshifted region